Centers

Center for Hypertension and Precision Medicine

CORE FACILITIES

In addition to the equipment available in their individual laboratories, faculty and trainees in our Department have access to additional equipment and resources that are integrated under a number of core facilities through the Center of Hypertension and Personalized Medicine.

Specifically, the Center for Hypertension and Precision Medicine manages and provides access to:

Genomic Core, which contains all the necessary equipment for genotyping, functional assays, mutation detection and positional cloning, such as StepOnePlus鈩� 96-well Real-Time PCR System (AB-biosystem); digital recording system for agarose electrophoretograms (Gel logic-100 imaging system); Applied Biosystems (ABI) 7500Fast qPCR machine and Agilent 2100 Bioanalyzer, among others.

Bioinformatics Core, which provides the infrastructure for the analysis and visualization of large datasets, with an emphasis on various sequencing data and promotes individual researchers to tap the ever-growing data generated by new research technologies (RNA-Seq, ChIP-Seq, DNA-Seq, and Methyl-Seq).

Biochemical Core, which contains all the standard equipment necessary for all biochemical analysis from tissue/cell culture samples.

Microbiome Core,听 which is equipped with an Illumina MiSeq next generation sequencer that听can generate 15 Giga bases and enables pooling of 96 samples in a library for metagenomics analysis. The core provides 16S metagenomics services for internal and external users, including DNA extraction, library preparation, sequencing and data analysis from a variety of biological samples (feces, saliva, oral swabs, tissues) of rodent, fish and human origin. After sequencing, data (FASTQ files) is processed and provided to the user in a compressed folder that includes operational taxonomic unit (OUT) summary, diversity analysis, taxa summary, enriched taxa, predicted functional pathways and statistical analyses.听 The microbiome core also performs whole genome sequencing for cultured bacteria using Illumina Nextera XT library kit, with as little as 10ng of DNA.
For pricing and more information click here: Microbiota/Microbial Community Services.

Faculty and trainees in our department also have access to additional Centralized Core Resources in the College of Medicine and Life Sciences:

听Center for Advanced Microscopy and Imaging

听Flow Cytometry Core Facility

听Electron Microscopy and Histopathology

听Department of Laboratory Animal Resources (DLAR)

Resources Available in Other Colleges at the University of Toledo:

听Center for Drug Design and Development

听The University of Toledo Instrumentation Center

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EXERCISE RAT RESOURCES

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We have developed rat models of aerobic exercise endurance capacity because of the close association of this phenotype with disease risks. Via two-way artificial selection we have generated models for intrinsic capacity and response to training.听

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LOW AND HIGH CAPACITY RUNNERS

Intrinsic Aerobic Running Capacity Models (LCR and HCR): In 1996 we started artificial divergent selection for intrinsic aerobic endurance running capacity using genetically heterogeneous N:NIH stock of rats as a founder population (n = 168). Selection for low and high capacity was based upon distance run to exhaustion on a motorized treadmill using a velocity-ramped running protocol. At each generation, within-family selection was practiced using 13 families for both the low and high lines. A rotational breeding paradigm maintained the coefficient of inbreeding at less than 1% per generation.

LOW AND HIGH RESPONSE TRAINERS

Response to Training Models (LRT and HRT): In 2002 a selection program for response to training was started from a founder population of 100 male and 100 female rats from the genetically heterogenous N:NIH stock. Response to training was operationally defined as the difference in a test of maximal treadmill running capacity conducted before and after 8 weeks of treadmill exercise training. A selection and rotational breeding program between 10 families for the low and high lines was started as the initial step to develop divergent genetic models.

听Visit Dr. Lauren Koch's profile page.听You may also contact her at听Lauren.Koch2@utoledo.edu.听

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